【摘要】 目的探讨神经细丝蛋白NFPs的异常磷酸化在阿尔茨海默病AD发病中的作用及其分布和溶解特性。方法超速离心法及免疫印迹法。结果人大脑灰质中三种神经细丝亚基主要分布在非水溶性级分;与亨廷顿氏病患者相比,AD患者大脑灰质非水溶性级分三种NF亚基的含量均升高,且升高的NF-H和NF-M主要表现为异常过度磷酸化形式。结论NFPs的异常磷酸化和聚集参与了AD的发病过程。更多还原

【Abstract】 Objective To explore the role of abnormal modification of neurofilament proteins (NFPs)in the pathogenesis of Alzheimer disease(AD),and the characteristics of NFPs in distribution and solubility.Method ultra-centrifugation and Western blot were utilized to isolate and analyze NF subunits.Ultra-centrifugation and Western blot were utilized to observe the effect of abnormal modification of neuro fi-la ment proteins (NFPs)on the characteristics of NFPs in distribution and solubility in the pathogenesis of Alzheimer disease.Results In human brain gray matter,NF subunits were mainly detected in par-ticular fraction.Compared with Huntington’ s disease(HD),the content of NF subunits was increased in AD brain gray matter and the increased level of NF-H and NF-M were mainly in abnormal hyperphosphorylated form.Conclusion Abnormal phosphorylation and accumulation of NFPs are involved in pathology of AD brain.更多还原