【摘要】 目的:研究黑石耳（Dermatocarpon miniatum,DEM）多糖对氧自由基的清除作用及其对脂质氧化（LPO）的抑作用。方法:羟自由基（·OH）由Fenton体系产生,超氧自由基（O₂^-）由邻三酚自氧化法产生,通过比色法测定脂质过氧化反应（LPO）的终产物——丙二醛（MDA）的相对含量来反映黑石耳多糖对LPO的影响。结果:黑石耳多糖对·OH和O₂^-有较强的清除作用,达到50％清除率所需药物浓度EC₅₀分别为1.72mg/ml和2.12mg/ml;可降低LPO的终产物MDA的含量。结论:黑石耳多糖对氧自由基有清除作用,对脂质过氧化有抑制作用,其活性大小与剂量呈正相关。更多还原

【Abstract】 Objective:To study the effects of Dermatocarpon miniatum(DEM) polysaccharides scavenging oxygen radicals and inhibiting lipid peroxidation.Methods:OH was produced by Fenton reaction and O2 was produced by the oxidation of pyrogallol. The inhibition to LPO was determined by the colorimetry for testing the relative content of MDA.Results: DEM polysaccharides could scavenge oxygen radical.The amounts of scavenging 50% (EC50)·OH was 1.72 mg/ml,the EC50 for O2 was 2.12 mg/ml. DEM polysaccharides could also decrease the content of MDA. Conclusion: DEM polysaccharides has the effects of scavenging oxygen radicals and the inhibition to LPO. Its activities showed positive correlation with the amounts.更多还原