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肉苁蓉不同外植体愈伤组织诱导比较

Studies on Calluses Induced firom Various Explants of Cistanche deserticola

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【作者】 朱咏华陈彩艳唐前瑞罗泽民

【Author】 Zhong Yonghua (Hunan College of Traditional Chinese Medicine, Changsha 410004)Chen Caiyan, Tang Qianrui, Luo Zemin (Hunan Agricultural University,Changsha 410128)

【机构】 湖南中医学院药学院湖南农业大学理学院湖南农业大学理学院 长沙 410004长沙 410128长沙 410128

【摘要】 肉苁蓉不同外植体花器、肉质茎、幼芽、鳞片等愈伤组织诱导比较结果表明:子房、茎块的愈伤组织诱导率高、时间短,且愈伤组织的质地较好。愈伤组织的增殖继代培养的初步研究表明:以B5为在本培养基,附加1mg/L IBA和2mg/L 6-BA效果较好。

【Abstract】 Calluses were induced from various explants (flower,stem,bud and squama) of Cistanche deserticola Y.C.Ma and compared with each other. The result showed that the inducement rate of ovary and stem were higer than that of other explants which the inducement time was short and the characters of calluses were good.The effect of basal culture medium added hormone on callus reproduction was also studied.The result showed that B5 culture medium added 1 mg/L IBA and 2 mg/L 6-BA was optimal.

【关键词】 肉苁蓉组织培养外植体愈伤组织诱导
【Key words】 Cistanche deserticolaTissue cultureExplantInducement
【基金】 国家中医药管理局资助
  • 【文献出处】 中药材 ,Journal of Chinese Medicinal Materials , 编辑部邮箱 ,2001年04期
  • 【分类号】S567.2
  • 【被引频次】18
  • 【下载频次】185
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