【摘要】 目的观察InsP6 及Ins对荷瘤小鼠不同器官中丙二醛(MDA)含量和红细胞中超氧化物歧化酶(SOD)的影响。从自由基角度探讨了其抑瘤机理。方法小鼠接种S180 细胞 ,次日腹腔连续注射不同剂量InsP6 及其与Ins的混合物 ,对照组注射等量生理盐水。10d后处死动物 ,计算抑瘤率 ;TBA法测量不同器官中MDA含量 ;NBT法测定红细胞中SOD活性。结果50mg/kg、100mg/kg、200mg/kgInsP6 抑瘤率分别为44.7 %、52.6 %、49.3 %。相应剂量的InsP6 和Ins的混合物(质量比1 :1)抑瘤率分别为28.1 %、36.5 %、22.1 %。药物治疗后 ,小鼠红细胞中被降低的SOD活性基本恢复至正常值 ,混合药物作用明显 ,其中50mg/kg、100mg/kg 剂量组较荷瘤对照分别增加了25.5 %、30.5 %。MDA含量的变化也因不同器官而异 :与荷瘤对照相比 ,给药后肿瘤内部MDA含量相对增高 ,InsP6 单独作用效果明显 ,100mg/kg剂量InsP6 使MDA含量增高48.1 %、脑组织中MDA含量变化不明显 ,荷瘤对照组胸腺中MDA与正常对照相比降低20.9 % ,治疗使MDA水平基本回升至正常。In sP6 作用显著 ,分别使其增高了33.5 %、30.3 %、37.2 %。结论InsP6 及其与Ins混合物能降低肿瘤细胞的增殖速度、使免疫祖细胞正常分化分裂 ,提高SOD活性 ,具剂量依赖关系。InsP6 具有最佳剂量100mg/kg ,而Ins更多还原

【Abstract】 Objectve To observe the effects of InsP6 and Ins on malonyl dialdehyde (MDA)Content in several organs and supuroxide dismutase (SOD) activity in red cells of rats with S180 tumor model. To study their anticancer mechanism from the point of free radicals. Methods Implanted 1×10 7 /cell/ml sarmon cell, the rats were peritoneoscope injected continuously with different doses of InsP6 and the mixture of InsP6 and Ins for 10 days, and the control group was injected with equal volume normal saline. Calculating the rate of tumor inhibition. Using TBA method to detect the MDA content in several different organs. Using NBT method to determine SOD activity in red cells. Results NO significant toxicity as evaluated by body weight gain. After injected with 50mg/kg、100mg/kg、200mg/kg InsP6 and 25mg/kg InsP6+25mg/kg Ins、50mg/kg InsP6+50mg/kg Ins、100mg/kg InsP6+100mg/kg Ins for 10 days, the rats’ tumor weight was reduced about 44.7%、52.6%、51.8% and 28.1%、36.5%、22.1% comparing with tumor controls. SOD activity in red cells was increased significantly in rats treated with 25mg/kg InsP6+25mg/kg Ins(increased 25.5%,P<0.05)and 50mg/kg InsP6+50mg/kg Ins (increased 30.5%,P<0.05).At the same time the changes of MDA content were distinct according to different organs. In contrast to control, tumor MDA content in treated rats was increased about 48.1% by 100mg/kg InsP6(P<0.05);Brain MDA content changed insignificantly; thymus MDA content of tumor control were decreased 20.9% comparing with normal control, and rats injected with different doses of InsP6 showed 33.5%、30.3%、37.2% rise in MDA content,almost returning to natural standard. Conclusion It suggests InsP6 and Ins may play a role as anticancer agents by increasing SOD activity,which can reduce free radicals.更多还原