【摘要】 肉桂酰辅酶A还原酶 (CCR)负责催化木质素单体生物合成中最重要的代谢反应 ,它将类苯丙酸类代谢物转移到木质素的合成途径中。为了更好地了解木质素在小麦生长发育中的作用 ,从小麦 (TriticumaestivumL .cv .H4 5 64)中克隆了两个肉桂酰辅酶A还原酶的cDNA。相似性和进化关系的分析表明这两个cDNA片段分别属于不同的肉桂酰辅酶A还原酶 ,这两个cDNA片段分别命名为W_cr6和W_cr19。RT_PCR和Northern杂交结果证明 ,W_cr6基因主要在小麦的茎和叶中表达 ,W_cr19基因主要在根和茎中表达。上述结果表明在小麦的基因组中至少存在两类肉桂酰辅酶A还原酶基因。更多还原

【Abstract】 Cinnamoyl_CoA reductase (CCR) is responsible for the first committed reaction in monolignol biosynthesis, which diverts phenylpropanoid_derived metabolites into the biosynthesis of lignin. To gain a better understanding of the lignin biosynthesis in wheat development, two cDNAs encoding CCR were identified from wheat ( Triticum aestivum L. cv. H4564). DNA sequence analyses indicated that the two cDNAs represent two classes of CCR. RT_PCR and Northern blot hybridization demonstrated that one of them, W_cr6, was expressed actively in stem and leaf tissue, the other one, W_cr19, was expressed in root and stem tissue. The results suggested that there are at least two genes encoded for CCR existing in wheat genome.更多还原