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钙-钙调素对小麦hsp26基因表达的影响
Involvement of Calcium-calmodulin in the Expression of hsp26 Gene in Wheat
【Abstract】 The treatment of wheat ( Triticum aestivum L.) seedlings with CaCl 2 increased mRNA levels of hsp26 gene at 37 ℃ for 1 h, while that with Ca 2+ chelator EGTA decreased the expression of the hsp26 gene. The expression of the wheat calmodulin gene CaM12 is rapidly upregulated in wheat seedlings by heat shock at 37 ℃. The heatinduced upregulation of CaM12 occurred earlier than that of the hsp26 gene did. Calmodulin antagonist N6aminohexyl5chloro1naphthalenesulfonamid (W7) inhibited the expression of the hsp26 gene in wheat seedlings at 37 ℃ for 1 h. These results implied that the calciumcalmodulin is probably involved in the signal transduction of the hsp26 gene expression induced by heat shock.
【关键词】 热激蛋白;
钙-钙调素;
基因表达;
小麦;
【Key words】 heat shock protein; calcium-calmodulin; gene expression; wheat;
【Key words】 heat shock protein; calcium-calmodulin; gene expression; wheat;
【基金】 国家自然科学基金 (3 9770 0 75 );国家重点基础研究专项经费 (G19990 1170 0 )资助项目&&
- 【文献出处】 植物学报 ,Acta Botanica Sinica , 编辑部邮箱 ,2001年07期
- 【分类号】Q943.2
- 【被引频次】25
- 【下载频次】176