【摘要】 利用PCR方法分别构建了烟草花叶病毒 (TMV)中一个推测为复制酶的 5 4_kD蛋白基因 (5 4K)缺失N端、C端和仅余基因中部 2 6 1bp的 3个缺失突变体 ,与野生型 5 4K一起克隆入植物中间载体p2 0 8,并通过根癌土壤杆菌(Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化烟草 (NicotianatabacumL .)。用TMV侵染转基因植物的R0 代和R1代 ,结果显示这 3个缺失突变体均能介导对TMV的抗病性更多还原

【Abstract】 Three deletion mutants of tobacco mosaic virus (TMV) 54_kD putative replicase gene (54K) were obtained by PCR, and cloned into plant expression vector p208, then transformed into Nicotiana tabacum L. cv. SR1 by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated transformation. All the transgenic plants with the N_terminal deletion mutant, the C_terminal deletion mutant and the only 261 nucleotides region from the central part of the 54K ORF showed significant resistance against TMV.更多还原