【摘要】 比较新鲜血液经荧光抗体标记后 ,当日或过夜后流式细胞仪检测免疫表型的差异 ,并采用过夜后流式细胞仪观察健康个体 4d内这些免疫指标的波动。CD4+ %、CD8+ %、HLA -DR+ %、CD8+ HLA -DR+ %用两种方法检测无显著性差异 (P =0 .841,P =0 .90 2 ,P =0 .0 6 0 ,P =0 .86 7) ;CD2 5 + %、CD4+ CD2 5 + %有显著性差异 (P =0 .0 2 1,P =0 .0 11)。表明检测CD4、CD8、HLA -DR抗原 ,可采用当日或过夜后流式细胞仪术检测 ;检测CD2 5抗原 ,用当日流式细胞较好。健康个体 4d内淋巴细胞亚群绝对值变异系数波动范围为 3.9%～ 2 5 .2 % ,百分率变异系数为 2 .8%～ 2 2 .2 % ;淋巴细胞表型绝对值变异系数波动范围为 1.5 %～ 45 .3% ,百分率变异系数为 2 .8%～ 40 .0 %。建议在对患者的免疫监测过程中 ,应考虑免疫指标的正常波动。更多还原

【Abstract】 To compare the results of lymphocyte subsets and phenoty pes in healthy individuals measured by flow cytometry between the same day and o vernight procedures and to observe changes of these parameters within 4 days in healthy adults with overnight procedure. There were no significant differences i n the levels of CD4~+%、CD8~+%、HLA-DR~+%、CD8~+HLA-DR~+% between the same day and overnight procedures (P=0.841,P=0.902,P=0.060,P=0.867)? but there were significant differences in levels of CD25~+%、CD4~+CD25~+% between the two p rocedures (P=0.021,P=0.011);CD4 and CD8 subsets and HLA-DR phenotypes can be mea sured by flow cytometry either with the same day or overnight procedure; but the same day procedure for measurement of CD25 is more stable. The CV ranges of the numbers and percentages of lymphocyte subsets were 3.9?25.2% and 2.8?22.2% re sp ectively. The CV ranges of numbers and percentages of lymphocyte phenotypes were 1.5?45.3% and 2.8?40.0%. We suggest that in survey of immune parameters of pa ti ents, normal ranges of changes of lymphocyte subsets and phenotypes should be ta ken into account in evaluation of patient’s immune status.更多还原