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肝片形吸虫长菲策吸虫及日本血吸虫DNA的多态性检测
Detection of DNA polymorphism among Fasciola hepatica,Fischoederius elongates and Schistosoma japonicum
【摘要】 本试验用随机扩增 DNA多态性技术对肝片形吸虫、长菲策吸虫和日本血吸虫虫体总 DNA的多态性进行检测 ,结果筛选出三条具有种鉴别意义的 RAPD谱带。这些特征性条带可望标记成探针用于粪便学检查中虫卵的鉴定
【Abstract】 The polymorphic DNA of Fasciola hepatica Fischoederius elongates , and Schistosoma japonicum were detected with RAPD in this assay. After amplification with six primers, three species specific RAPD patterns were found, which could be used as a probe for identification of eggs in feces examination.
【关键词】 肝片形吸虫;
长菲策吸虫;
日本血吸虫;
RAPD特征条带;
【Key words】 Fasciola hepatica; Fischoederius elongatus; Schistosoma japonicum; species specific RAPD patterns;
【Key words】 Fasciola hepatica; Fischoederius elongatus; Schistosoma japonicum; species specific RAPD patterns;
【基金】 欧洲联盟资助项目 ( INCD-DCIC-18-CT95 0 0 0 1)
- 【文献出处】 中国兽医杂志 ,Chinese Journal of Veterinary Medicine , 编辑部邮箱 ,2001年12期
- 【分类号】S852.735
- 【被引频次】2
- 【下载频次】47