【摘要】 采用反相液相色谱法测定饲料级、预混合饲料和配合饲料中的金霉素含量方法的研究。色谱柱为 C18预柱及分析柱 1 50× 4.6mm,5μm;流动相为 0 .0 1 mol/L草酸溶液 -乙腈 -甲醇 ( 1 0∶ 3∶ 2 ) ;紫外检测器 ,检测波长为 360 nm;柱温 2 5℃。结果为在 0 .2～ 50μg/ml之间呈现良好的线性关系 ,相关系数 r为 0 .9997,3个不同浓度样品加样回收率 ( n=5)分别为 95.96% ,98.32 % ,95.41 %。本法简便 ,快速 ,准确度和精密度较高 ,适用于各种饲料中金霉素含量的测定更多还原

【Abstract】 A high performance liquid chromatographic (HPLC) method was developed for determination of Chlortetracycline (CTC) in premix and formulated feed. CTC was extracted with Acid Acetone solution, extracts were adjusted pH 1.0~1.2 to stability with HCl, and shaked and centrifuged, extracts was filtered and quantitated by an external standard curve of method. The HPLC using a C 18 precolumn filter, and a steel column and UV detector at 376 nm wavelength. The mobile phase was used 0.01 mol/L Oxalic acid Acetonitrile Methanol (10∶3∶2). The average recovery of CTC from feeds with three different concentrations was 95.96%,98.32%,95.41% respectively. A good linear response was obtained for CTC standard from 0.2 to 50.0 ug/ml, and the correlation coefficient was 0.9997.更多还原