【摘要】 选用 DEAE-纤维素离子交换柱对双峰驼精清进行分离 ,各层析组分用于大鼠垂体组织体外培养 ,并给母驼肌注有活性的组分 ,以 RIA法测定垂体组织培养液和母驼血浆中 LH及 FSH的浓度。结果表明 ,驼精清经 DEAE-纤维素柱分离后可得到 5个蛋白组分 ( L1～ L5) ,其中 L3在大鼠垂体培养时引起 LH的释放明显增加 ( P<0 .0 5) ,FSH则无明显变化。给卵巢上无大卵泡发育的母驼肌注有活性的组分 L3 ,可引起血浆中LH明显升高 ,FSH也无变化。由此表明 ,L3在体内外试验中均具有引起 LH释放的生物活性 ,它可能是诱导排卵的活性因子或其成分之一。更多还原

【Abstract】 The seminal plasma of camel was separated by DEAE cellulose column,five fractions were obtained,and biological activity of each fraction was analyzed by rat pituitary tissue culture in vitro and by their intramuscular injection to the camel in vivo .The concentrations of LH and FSH in the pituitary culture media before and 6 h after addition of each fraction and in the blood plasma samples collected from the injected camels immediately before and hourly after injection were measured by RIA.The results showed that the third fraction(L3) had biological potential to stimulate the release of LH significantly in vitro from (11.82±1.77) IU/L to (25.63±3.84) IU/L after the addition of L3 in the culture media.The concentration of LH in the blood plasma before and 6 h after the injection were(6.43±0.14) μg/L and (15.50±5.64) μg/L respectively.However,the concentration of FSH did not show any significant changes both in vitro and in vivo .更多还原