【摘要】 【目的】研究抗人食管癌单克隆抗体G9(McAbG9)在荷瘤裸鼠体内对食管癌组织的定位作用。【方法】采用氯胺 T法制备标记物12 5I G9,纯化后腹腔注射 ,分别于 2 4、48、72h取多种器官 /组织测放射性 ,计算每克组织摄取放射性的百分比(%ID/ g)及肿瘤与非瘤组织的比T/NT ,并取 72h的肿瘤组织进行放射自显影分析。【结果】12 5　I G9肿瘤组织的放射性均较其它器官 /组织高 (血液除外 ) ,48h组最高 ,T/NT值在 2～ 7之间 ;放射自显影显示肿瘤组织有大量放射性浓集 ,肿瘤边缘较中心部位明显。【结论】12 5　I G9能在荷瘤裸鼠体内定位于食管癌部位 ,单抗G9具有很好的导向作用。更多还原

【Abstract】 Objective To study the location effect of the McAb G9 to human esophageal carcinoma in tumor bearing nude mice. Methods 125 I-G9 was prepared by Chloramine-T method. The distribution of 125 I-G9 was detected at different time (24, 48, and 72 h) after peritoneal injection. The percentage of the injected dose per gram of tissue(%ID/g) and the ratio of Tumor/non-Tumor were calculated. Results The distribution of 125 I-G9 in tumor at the third day was showed by autoradiography obviously higher than in other organ/tissue (except blood) and the highest is at the 48 h. The T/NT values are 2-7. The autoradiography indicated that radioactivity concentrated in tumor tissue. The concentration in tumor edge is more obvious than in the center. Conclusion 125 I-G9 has a considerable targeting activity and can well locate in esophageal carcinoma tissue in tumor bearing nude mice.更多还原