【摘要】 组织因子是机体外源性凝血途径的起始因子 ,研究证实它与生物材料在机体内诱发的血栓形成密切相关 ,我们以前进行的体外研究发现用组织因子的抑制因子 组织途径抑制因子 (tissuefactorpathwayinhibitor,TFPI)处理生物材料可以明显延长在其表面发生的凝血时间。本文进一步探讨了重组TFPI处理涤纶人工血管材料对其植入动物血管内后诱发血栓的影响。本研究中将涤纶人工血管材料裁成细条并浸润于谷胱甘肽硫基转移酶 (glutathione S transferase ,GST)或GST TFPI融合蛋白溶液中置 4℃过夜 ,然后植入实验犬的双侧股动脉 ,随时记录股动脉的搏动情况 ,2h后取出材料进行血栓称重。我们的结果显示重组TFPI处理材料可以明显延长动脉闭塞时间及减少材料表面的血栓形成 ,这提示TFPI在改善生物材料血液相容性方面的巨大应用潜力。更多还原

【Abstract】 Tissue factor(TF) is the initiation factor of the extrinsic pathway of the blood coagulation cascade. Our previous study showed that its natural inhibitor, tissue factor pathway inhibitor(TFPI), could obviously prolong the blood coagulation time on the surface of biomaterials. In the present study, the effect of TFPI on the thrombogenicity of Dacron membrane in canine femoral artery was investigated. Dacron membrane was cut into strips and incubated with GST or GST-TFPI fusion protein at 4℃ over night. The strips were then implanted into canine femoral artery with the GST-TFPI group being put at the proximal end and GST group the distal end. The arterial embolism time was counted before the strips were taken out at 2 hours after implantation. Our result indicated that GST-TFPI treatment could dramatically prolong the arterial embolism time and decrease the thrombus weight formed on the membrane compared to the control group. It suggested that TFPI have a great potential to be used as an anticoagulant for improvement of the haemacompatibility of biomaterial.更多还原