【摘要】 神经氨酸酶 (唾液酸酶 )与红细胞发生生物化学作用 ,分别改变其作用时间和二者的相对作用剂量 ,以达到不同程度地去掉红细胞的表面电荷。用文宗曜、严宗毅等[1] 最近提出的一种测量红细胞膜剪切弹性模量及表面粘度的新方法 新型激光衍射法 ,分别测量经过各种处理的红细胞的小变形指数 (DI) d 和变形恢复过程即松弛过程中变形恢复到最大值 (DI) max一半的时间t0 .5(即变形恢复半时间 ) ,将测得的结果分别代入红细胞膜的剪切弹性模量 (E)公式和红细胞面粘度 (μm)公式。计算出经过各种处理后的红细胞的膜剪切弹性模量和表面粘度 ,并与正常对照组的相应参数作比较 ,发现二者之间存在明显差异 ,此差异随处理程度的增加而增大 ,表现出明显的量效关系。因此有力地证明了神经氨酸酶对红细胞作用后使其膜剪切弹性模量增大 ,表面粘度增大 ,同时也体现了新型激光衍射法在测量红细胞膜的剪切弹性模量和表面粘度时有很好的灵敏性 ,它与国外文献所报道的微吸管法和流室法测得的结果相近 ,但操作方便 ,省时、省力、造价低 ,因此具有更广泛的应用范围。更多还原

【Abstract】 Neuraminidase and RBCs have a biochemical interaction. We changed the time of treatment and the dose of neuraminidase to remove the surface charge of RBCs in different degrees. Using a new ektacytometry, we measured the small deformation index ( DI) d and the half time (t 0.5 ) when the deformation index recovered to the half of maximum during the process of relaxation for every treated sample. ( DI) d and (t 0.5 ) were respectively put into the RBC membrane shear elastic modulus (E) formula and the membrane viscosity (μ m) formula which were put forward by Wen Zongyao and Yan Zongyi et al [1] . There were obvious differences between the treated and the control RBCs in their two parameters ( E and μ m). The differences became great when the degree of treatment increased and there was a quantity effect relation between them. This study showed that RBC′s E and μ m increased after the treatment with neuraminidase. It confirmed that the new ektacytometry had a good sensitivity in measuring E and μ m because its results were close to the results reported in the foreign articles and measured by micropipette and flow channel. Since this method is convenient and cheap, it will have a wide range of application.更多还原