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苏云金杆菌可溶性增效物质的增效活性

Properties of Water-Soluble Potentiator Produced by Bacillus thuringiensis in Supernatant

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【作者】 杨自文王开梅张志刚镇达吴继星钟连胜岳书奎

【Author】 YANG Ziwen, WANG Kaimei, ZHANG Zhigang, ZHEN Da WU Jixing, ZHONG Liansheng, YUE Shukui (Hubei Biopesticide Engineering Research Center, Wuhan 430064, China)

【机构】 湖北省生物农药工程研究中心东北林业大学森林资源与环境学院 武汉430064武汉430064武汉430064

【摘要】 浓缩上清液分别与发酵液、菌浆混配可获得毒力为 80 0 0IU/ μl和 1 6 0 0 0IU/ μl的悬浮剂。增效粉同原粉按不同比例混配 ,对甜菜夜蛾增效倍数最高可达 1 1 1 4倍。当MP 342原粉和增效粉以质量分数 1∶2~ 4的比例混合时 ,可生产 32 0 0 0IU/mg和 6 40 0 0IU/mg的粉剂产品。增效粉经过 54℃处理 3d后增效作用维持在初始增效活性的 70 %左右 ,不再继续下降。贮存 1 3个月的液剂上清液增效倍数较起始增效倍数下降近 50 %。增效物质粉态的稳定性好于液态的稳定性

【Abstract】 The stability and the activity of water soluble potentiator were carried out in present study. Liquid formulation with efficacy of 8000IU/μl and 16000IU/μl could be made by mixing concentrated supernatant with fermentation broth and sludge, respectively. The toxicity to Spodoptera exigua could increase 11 14 times when mixing MP 342 technical powder with potentiator powder at the ratio of 1∶10. 32000IU/mg and 64000IU/mg powder preparations were obtained at the mixing ratio of 1∶2-4 of the technical powder and potentiator powder. The powder potentiator was relatively stable, it maintained 70% of original activity after 14d treatment at 54℃. The potentiator activity of supernatant in liquid formulation decreased about 50% after 13 months storage at room temperature.

【基金】 湖北省科委重点科技攻关项目 (95 1 P 0 90 2 )
  • 【文献出处】 中国生物防治 ,Chinese Journal of Biological Control , 编辑部邮箱 ,2001年04期
  • 【分类号】S476.11
  • 【被引频次】6
  • 【下载频次】80
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