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苏云金杆菌可溶性增效物质的增效活性
Properties of Water-Soluble Potentiator Produced by Bacillus thuringiensis in Supernatant
【摘要】 浓缩上清液分别与发酵液、菌浆混配可获得毒力为 80 0 0IU/ μl和 1 6 0 0 0IU/ μl的悬浮剂。增效粉同原粉按不同比例混配 ,对甜菜夜蛾增效倍数最高可达 1 1 1 4倍。当MP 342原粉和增效粉以质量分数 1∶2~ 4的比例混合时 ,可生产 32 0 0 0IU/mg和 6 40 0 0IU/mg的粉剂产品。增效粉经过 54℃处理 3d后增效作用维持在初始增效活性的 70 %左右 ,不再继续下降。贮存 1 3个月的液剂上清液增效倍数较起始增效倍数下降近 50 %。增效物质粉态的稳定性好于液态的稳定性
【Abstract】 The stability and the activity of water soluble potentiator were carried out in present study. Liquid formulation with efficacy of 8000IU/μl and 16000IU/μl could be made by mixing concentrated supernatant with fermentation broth and sludge, respectively. The toxicity to Spodoptera exigua could increase 11 14 times when mixing MP 342 technical powder with potentiator powder at the ratio of 1∶10. 32000IU/mg and 64000IU/mg powder preparations were obtained at the mixing ratio of 1∶2-4 of the technical powder and potentiator powder. The powder potentiator was relatively stable, it maintained 70% of original activity after 14d treatment at 54℃. The potentiator activity of supernatant in liquid formulation decreased about 50% after 13 months storage at room temperature.
【Key words】 Bacillus thuringiensis; supernatant; potentiator; vacuum evaporation; spray drying; bioassay;
- 【文献出处】 中国生物防治 ,Chinese Journal of Biological Control , 编辑部邮箱 ,2001年04期
- 【分类号】S476.11
- 【被引频次】6
- 【下载频次】80