【摘要】 1 甲基 4 苯基 1,2 ,3,6 四氢吡啶 (MPTP)对C57BL小鼠黑质多巴胺能神经元具有选择性的毒性作用 ,导致小鼠出现类似帕金森病的症状。利用抑制消减杂交技术分析MPTP对小鼠黑质基因表达的影响 ,获得了一系列差异表达基因片段 ,其中包括多巴胺转运体与甘氨酸受体两种与神经递质的功能与周转密切相关的基因。通过Northern杂交发现 ,这两种基因的表达在给药组小鼠的黑质中均下降 ,而在纹状体中多巴胺转运体基因的表达没有明显变化 ,甘氨酸受体基因的表达则有一定程度的增加。更多还原

【Abstract】 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) treatment of C57BL mice can result in selective loss of dopaminergic neurons in substantia nigra and a symptom similar to Parkinson’s disease. The gene expressions in substantia nigra of MPTP-treatment C57BL mice were analysed by the method of suppression subtractive hybridization(SSH). Dozens of differentilly expressed gene fragments were cloned, including the gene fragments of dopamine transporter and glycine receptor. The mRNAs of these two genes in substantia nigra and striatum were detected by Northern hybridization. It was found that the expressions of both genes were decreased in the substantia nigra of MPTP-treated mice, the expression of dopamine transporter gene showed no significant difference and the expression of glycine receptor gene was increased in striatum of MPTP-treatment mice.更多还原