【摘要】 根据伪狂犬病病毒 (PRV)Rice株gE基因的序列设计并合成了 1对引物 ,以我国PRV地方毒株广东株的基因组DNA为模板 ,通过PCR方法获得了一大小约 1 6kb的DNA片段 ,并将其克隆到pMD18_T载体上进行测序 .序列测定结果显示 ,该片段长 16 6 5bp ,编码 5 5 5个氨基酸 ,与PRVRice株gE基因的核苷酸序列同源性为 97 7%,氨基酸序列同源性为 95 9%更多还原

【Abstract】 A fragment about 1.6 kb was amplified by PCR technique from the genome DNA of PRV Guangdong strain,using primers designed and synthesized according to the gE gene fragment exclude signal peptide of PRV Rice strain. This PCR product was then cloned into pMD18-T vector and sequenced. The sequencing result showed that the PCR product spanned 1 665 bp,encoding 555 amino acids. The homology of DNA sequence and amino acid sequence between it and gE gene of Rice strain was 97.7% and 95.9% respectively.更多还原