【摘要】 目的　运用表达序列标签 (ExpressedSequenceTag ,EST)技术快速、经济地发现日本血吸虫 (中国大陆株 )新基因。方法　随机挑取日本血吸虫 (中国大陆株 )成虫cDNA文库单个重组克隆进行部分测序以获得EST ,获得的EST通过BLAST程序同EMBL寄生虫数据库和GenBank数据库进行比较及同源性分析。结果　本研究共随机挑取日本血吸虫 (中国大陆株 )成虫cDNA文库单个重组克隆 2 0 0个 ,获得了 76个有EST价值的序列 ,其中 7.9%为日本血吸虫已知序列 ,5 .3 %为日本血吸虫同源序列。曼氏血吸虫或其他生物的同源序列占有EST价值的序列的 2 2 .4 % ,未知序列占 5 9.2 %。在获得的76个有EST价值的序列中 ,66个成功在GenBankdbEST中登录。通过同源性分析 ,发现了一些令人感兴趣的基因。结论　EST方法有助于快速、经济地发现日本血吸虫 (中国大陆株 )成虫新基因。更多还原

【Abstract】 Aim To rapidly and economically obtain the new genes of adult stage Schistosoma japonicum (Chinese strain) by expressed sequence tag (EST) strategy.Methods A directional cDNA library constructed from Schistosoma japonicum (Chinese strain ) adult stage RNA was used to generate expressed sequence tags(ESTs) and the ESTs obtained were compared against EMBL-parasites database and GenBank datebase by BLASTn and BLASTx.Results A total 200 phage clones were randomly selected for generating expressed sequence tags(ESTs).From these clones,we obtained 76 EST-quality sequence.A total of 7.9% of these EST-quality sequences were identified suquence of Schistosoma japonicum,while 5.3% were putatively identified sequence of Schistosoma japonicum.A total of 22.4% of these EST-quality sequences were putatively identified sequence of Schistosoma mansoni or other organisms,and 59.2% which had no matches in the database were classified as unknow sequence.Among these EST-quality sequences,66 ESTs were successfully submitted to the dbEST at GenBank.In addition,some interesting genes were found.Conclusion Partial cDNA sequencing to get expressed sequence tags(ESTs) was a rapid and economical method to discover new genes of adult stage Schistosoma japonicum(Chinese strain).更多还原