【摘要】 目的　构建携带沙眼衣原体L2型主要外膜蛋白 (MOMP)基因的真核表达载体 ,为沙眼衣原体的DNA疫苗的研究提供材料。方法　用PCR技术扩增L2型沙眼衣原体MOMP基因片段 ,再将其定向插入到真核表达载体 pcDNA3多克隆位点中 ,构建成重组表达质粒。并用酶切分析、PCR扩增及部分序列测定等方法对重组质粒进行了鉴定。结果　MOMP基因被正确地克隆到真核表达载体 pcDNA3中。测序结果同文献报道的序列一致。 结论　真核细胞表达载体 pcDNA3 -MOMP的成功构建 ,为进一步开展沙眼衣原体的DNA疫苗的研究奠定了基础。更多还原

【Abstract】 Aim To construct an eukaryotic expression vector carrying the Chlamydia trachomatis major outer membrance protein (MOMP) gene.Methods The MOMP gene was amplified from the genome of the Chlamydia trachomatis serovar L2 by PCR and recombined with plasmid pcDNA3.The recombinant plasmid pcDNA3-MOMP was verified with restriction analysis,PCR and partial sequencing.Results MOMP gene was cloned correctly into vector pcDNA3.The resultant sequence is completely consistent with the published sequence.Conclusion The eukaryotic expression vector pcDNA3-MOMP was constructed successfully for research on DNA vaccine of Chlamydia trachomatis.更多还原