【摘要】 目的研究反义硫代磷酸寡核苷酸（ＡＳ－ＯＤＮ）抑制人肝癌细胞耐药株（ＳＭＭＣ－７７２１／ＡＤＭ）ＭＲＰ 基因表达的作用。方法用人工合成互补于ＭＲＰ 基因ｍＲＮＡ特定片断的反义硫代磷酸寡核苷酸，以脂质体Ｌｉｐｏｆｅｃｔａｍｉｎｅ为载体转染ＳＭＭＣ－７７２１／ＡＤＭ细胞株，用ＲＴ－ＰＣＲ测定ｍＲＮＡ表达，流式细胞技术测定细胞膜ＭＲＰ１蛋白Ｐ１９０表达及ＭＴＴ法检测细胞对柔红霉素（ＤＮＲ）和阿霉素（ＡＤＭ）的敏感性。结果 ＡＳ－ＯＤＮ能抑制 ＭＲＰｍＲＮＡ和其蛋白 Ｐ１９０表达，提高细胞对 ＤＮＲ和 ＡＤＭ的敏感性。结论① ＡＳ－ＯＤＮ能降低 ＭＲＰ基因表达；② ＭＲＰ介导的多药耐药（ ＭＤＲ）是ＳＭＭＣ－７７２１／ＡＤＭ耐药的重要机理之一。更多还原

【Abstract】 Objective To investigate the effect of phosphorothioate antisense oligonncleotides (AS-ODN) on sup pressing multidrug resistance-associated protein gene(MRP) in human drug-resistant hepatocellular carcinoma cell line (SMMC-7721/ADM). Methods Cell line was transfected with a synthetic S-ODN complementary to the coding region of MRP mRNA, Lipofectamine acting as carrier. The drug sensitivity was measured by MTT assay. The expression of MRP mRNA was detected by RT-PCR and the expression of P190, was detected by flow cytometry. Results AS-ODN inhibited expression of MRP mRNA and P190 and promoted sensitivity to daunorubicinum and adriamycinum. Conclusion AS-ODN can reduce the expression of MRP gene. MDR caused by MRP is an important cause of multidrug resistance of SMMC-7721/ADM.更多还原