【摘要】 培矮 64S是由光敏核不育水稻农垦 5 8S作不育基因供体转育而成的 ,但其育性表现为较强的温度敏感性。从培矮 64S与常规稻品种农垦 5 8杂交后代 F2 中获得了典型的光敏雄性不育株 ,其可育株与不育株比例为 3∶ 1;证明控制培矮 64S育性的主效基因仍属光敏核不育基因。笔者在分析后认为 ,光敏核不育基因的高效表达对其所在的遗传背景有高度依赖性 ,在光敏核不育系选育中 ,在注重获得主效光敏不育基因的同时 ,还要注重对其高效表达的遗传背景的选择与改造。在利用克隆的光敏核不育基因进行分子育种时也要高度重视对转基因亲本遗传背景的选择更多还原

【Abstract】 Peiai64S, an indica male sterile rice with male fertility alternation trait under different environments, is selected from the offspring of indica rice cross with Nongken58S. Nongken58S, a japonica photoperiod sensitive genic male sterile rice (PGMS) and come from a natural mutant plant of normal japonica rice Nongken58, is used as a male sterile gene honor of Peiai64S. But Peiai64S is not a typical PGMS rice, the male fertility is more sensitive to temperature like a thermo sensitive genic male sterile rice (TGMS). We selected typical PGMS plants in F 2 population of Peiai64S×Nongken58, the fertility plants and sterile plants distribution is near 3∶1. The sterile gene is conformed to one pair gene segregation model. The result indicates the chief male sterile gene in Peiai64S isn’t other, it is the PGMS gene come from Nongken58S. The genetic background affected PGMS gene effective express. This suggested that, we ought to focus on optimizing the genetic background of PGMS gene in PGMS rice breeding, and select an idea genetic background as transgenic background in molecular breeding.更多还原