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利用RAPD标记鉴定绿豆组植物种间亲缘关系
Study on Relationships Among Species in Mungbean Group Using RAPD Markers
【摘要】 利用RAPD分子标记技术,对56个绿豆组品种(系)进行亲缘关系研究。在选用的45个随机引物中,均发现有不同的扩增产物。通过聚类分析将它们分成黑吉豆、野生绿豆和栽培绿豆3个种群。在栽培绿豆中又可分出印度抗豆象、巴基斯坦抗黄花叶病毒、中国Ⅰ组、中国Ⅱ组和亚蔬绿豆5个类型组。获得绿豆组3个豆种及其代表品种的独特标记,为今后食用豆种质资源鉴定与分类和遗传分析提供理论依据。
【Abstract】 RAPD markers were used to study relationships among species in mungbean group. Fifty six accessions, including 52 mungbean ( Vigna radiata ), 2 black gram (V. mungo )and 2 wild types ( V. sublobata) were examined with 45 random 10-mer primers which produced different RAPD bands among them. These mungbean accessions can be classified into 3 species, i. e. , the black gram species, wild mungbean species and cultivated mungbean species. In addition, the last species can be further divided into 5 groups, namely, the Indian resistant bruchid group, the Pakistani resistant yellow mosaic virus group, Chinese I group, Chinese I group and AVRDC group. The peculiar markers of 3 species and advanced varieties were generated and provided a theoretical background for the evaluation, taxonomy and genetic identity of food legumes germplasm resources.
- 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,2001年02期
- 【分类号】S522
- 【被引频次】25
- 【下载频次】148