【摘要】 影响小麦遗传转化的因素很多，如基因型、转化方式、表达载体上的启动子和筛选标记，以及转化后的有效筛选。为此，对小麦遗传转化的主要因素进行了研究。结果表明，针对载体上的 ＮＰＴⅡ筛选标记基因．Ｇ４１８的适宜用量为 ２５～５０ｍｇ／Ｌ，而不能用卡那霉素和新霉素，针对载体上的Ｂａｒ基因筛选标记，ＰＰＴ或Ｂｉａｌｏｐｈｏｓ的适宜用量为 ３～５ｍｇ／Ｌ，针对载体上的 Ｈｐｔ筛选标记基因，潮霉素的适宜用量为 １００～１５０ｍｇ／Ｌ；从 ２５个小麦基因型中，筛选到了扬麦１５８和扬麦１０号等几个幼胚培养力比较高的基因型；选择培养基中选择剂浓度的合理变化对于获得转基因再生植株是有益的。更多还原

【Abstract】 Wheat transformation efficiency was closely related to several aspects such as donor geno- type, constructed plasmid and selection procedure after bombardment or co-cultivation. Several kinds of antibiotics, which were normally used in plant transformation for the selection genes of NPT Ⅱ, Bar and Hpt, were tested for the optimal concentrations in wheat transformation. Geneticin (G418) of 25- 50mg/L was only used for the selection of NPT Ⅱ, kanamycin or neomycin was not available. 3-5mg/L of phos- phinothricin(PPT) or bialophos can be used for the selection of Bar, 100~ 150mg/L of hygromycin for the selection of Hpt. Yangmai 158 and Yangmai 10 with high tissue culture response and good agronomic char- acteristics were screened from 25 potential Chinese wheat cultivars. The concentration changing of se- lectable agent in selection medium was necessary to obtain enough regeneration plantlets with strong root system.更多还原