【摘要】 目的 :探讨人白细胞介素 15的纯化工艺和进行活性鉴定。方法 :将rhIL 15工程菌大量扩增后通过包涵体提取、复性、凝胶过滤、阴离子交换层析、高效反相液相色谱等技术进行纯化。结果 :经纯化得到rhIL 15 ,其分子量为 14 4kD ,蛋白纯度为 99%以上 ,比活为 1× 10 7U/mg ,对NK细胞杀伤活性具有明显的促进作用。结论 :通过三步纯化方法可以获得高纯度、高回收率和高生物学活性的rhIL 15 ,且具有明显的促NK细胞杀伤活性。更多还原

【Abstract】 Objective:Studies on purification and bioactivity detection of recombinant human IL 15 protein.Methods:The rhIL 15 were purified from rhIL 15 producing DH5α bacteria the inclusion bodies extracted first and followed by gel filtration,ion exchange chromatography and reverse phase high performance liquid chromatography(RP HPLC).The bioactivities were detected by NK cytotoxicity.Results:Recombinant human IL 15(rhIL 15)was obtained with a purity of 99 percent.The N terminal amino acid sequence of the rhIL 15 was as same as the natural IL 15.The specific activity of the rhIL 15 is 1×10 7 U/mg,and the rhIL 15 significantly enhances NK cytotoxicity.Conclusion:Recombinant human IL 15 of high purity and bioactivity could be obtained by three steps of purification.The high quanity and quality of the rhIL 15 is important to both the laboratory research and the clinical trial.更多还原