【摘要】 目的 :比较不同地株微小按蚊核糖体DNA(rDNA)内转录间隔 2区 (ITS2 )序列差异。方法 :通过对PCR扩增rDNAITS2片段测序 ,比较其不同地株差异。结果 :对 6株微小按蚊测序比较 ,存在有微小按蚊A和C型。结论 :rDNAITS2序列差异可用于建立A、C型的分子鉴别方法更多还原

【Abstract】 Objective:To compare the sequence difference of the ribosomal DNA second internal transcribed spacer (ITS2) in the different strains of Anopheles minimus in Yunnan. Methods: The PCR amplified rDNA ITS2 sequences, to compare the variation of An. minimus . Results: The different strains of 6 populations were sequenced, which could be separate into Anopheles minimus A and C. Conclusion: The sequence difference of the rDNA ITS2 would be very useful for the developing a molecular approach to distinguish An. minimus A and C.更多还原