【摘要】 目的　将化学发光免疫测定 (Chemiluminescenceimmunoassay)方法应用于血清CEA测定 .方法　以L9(34 )正交设计确定化学发光免疫测定方法检测血清CEA的最适实验条件 ,并对方法的特异性、敏感性和重复性进行评价 .结果　化学发光免疫测定方法测定血清CEA的最适实验条件为 :CEAMcAb包被质量浓度 10mg/L ,ABEI CEAMcAb结合物使用发光强度为 1.0× 10 4 mV ,Hemin浓度为 5 μmol/L ,H2 O2 质量分数为 0 .3% .结论　化学发光免疫测定方法检测血清CEA特异性强、重复性好、敏感性超过ELISA和RIA方法 ,且与ELISA和RIA方法高度相关 .更多还原

【Abstract】 Objective Chemiluminescence immunoassay (CLIA)was applied in measuring serum CEA.Methods Orthogonal design L 9(3 4) was used in confirming the optimal experimental conditions of CLIA for serum CEA and the specificity, sensitivity and reproducibility of this method were evaluated.Results The optimal conditions of CLIA for serum CEA were as follows: 10?mg/L?CEA?McAb,1.0×10 4mV?ABEI CEA?McAb conjugate,5?μmol/L,Hemin,0.3%H 2O 2.Conclusion CLIA for serum CEA was more specific,reproducible,sensitive than ELISA and RLA and related to ELISA and RIA.更多还原