【摘要】 目的探讨地塞米松对人胶质瘤细胞有无诱导分化作用。方法 :将人胶质瘤细胞系 SHG- 44在含 1mg/ L 地塞米松、10 %小牛血清的 R16 40培养基中培养 48h。进行形态学观察和凝集实验 ,并计算分裂指数和Ag NOR个数 ,免疫组化法检测细胞胶质纤维酸性蛋白 (GFAP)表达量的变化。结果 :加入地塞米松组细胞贴壁更加牢固 ,胞体肥大 ,突起增多 ,梭形细胞由 87%下降到 41% ;凝集程度明显下降 ;分裂指数由 11.2 0 %降至 4.90 % ;Ag NOR明显萎缩、消失 ,数量也由 1.31个 /核减少到 0 .77个 /核 ;GFAP含量增加。结论 :初步表明地塞米松对人胶质瘤细胞具有诱导分化作用更多还原

【Abstract】 Objective: To study the effect of dexamethasone in differentiating human glioma cell line .Methods: The human glioma cell line was incubated with l mg/L dexamethasone in 1640 culture medium with 10% vitulary serum for 48 hours. The cell form was observed by contrapositive microscope,and PHA was used to induce the agglutination of these cells.Mitotic index and AgNOR amount was counted. The expression of GFAP was detected by immunocytochemisty.Rusults: SHG 44 cells incubated with dexamethasone adhered to plate firmly and its shape became astroid .The agglutination degree and mitotic index decreased significantly .The Ag NOR was atrophied and its amount decreased.The Immunocytochemistry showed the content of GFAP increased sign ficantly.Conclusion: Dexamethasone plays a role in differentiating human glioma cell.更多还原