【摘要】 目的　研究脑胶质瘤中IL 6 /IL 6R自分泌或旁分泌环路的存在和意义。方法　采用逆转录聚合酶链反应 (RT PCR)方法检测白细胞介素 6 (IL 6 )和IL 6受体 (IL 6R)基因在脑胶质瘤细胞株、脑胶质瘤组织标本和正常人脑胶质细胞中的表达。结果　 2株脑胶质瘤细胞株均有IL 6和IL 6R的表达 ,5 2例脑胶质瘤组织标本中有 38例 (73.1% )表达IL 6、4 4例 (84 .6 % )表达IL 6R、33例 (6 3.5 % )同时表达IL 6和IL 6R。人脑正常胶质细胞仅有IL 6基因弱表达 ,而无IL 6R表达。结论　脑胶质瘤可能存在IL 6 /IL 6R自分泌或旁分泌环路 ,此环路与肿瘤的恶性增殖有关。更多还原

【Abstract】 Objective To evaluate the significance of the autocrine of paracrine interleukin\|6/interleukin\|6 receptor loop in gliomas.Methods We detected IL\|6 and IL\|6R gene in adult cerebral tissue, cerebellar tissue, 2glioma cell lines and 52 human glioma tissue specimens with different malignant degree. A complementary DNA copy of total RNA was synthesized and amplified with specific primers using the reverse transcription polymerase chain reaction(RT\|PCR)method.ResultsThe experiments revealed that both C6 and SHG\|44 glioma cell lines expressed IL\|6 and IL\|6R. It was positive of IL\|6 gene in 38 samples (73.1%)and positive of IL\|6R gene in 44 samples (84.6%)respectively.There were 33(63.5%)glioma tissue specimens that coexpressed IL\|6 and IL6R gene. Moreover, the positive rate and intensity of the two gene expression was related to the malignant degree of gliomas. On the other hand, the expression of IL\|6 was weakly positive in adult cerebral and cerebellar tissue, but IL\|6R was negative.Conclusion Our results indicate that IL\|6 and IL\|6R probably play an important role in the genesis and developing of human glioma. Maybe there is IL\|6/IL6R autocrine or paracrine loop in gliomas.更多还原