【摘要】 目的　探讨 p1 5基因变异及其与脑胶质瘤的发生、恶性进展的关系。方法　利用 PCR和 PCR- based甲基化检测技术检测了 56例脑胶质瘤中 p1 5基因外显子 1缺失及 5′CPG岛甲基化情况。结果　 43例高级别的脑胶质瘤中 ,1 4例发生了 p1 5基因缺失 ( 32 .6% ) ,而 1 3例低级别的脑胶质瘤中无一例发生 p1 5基因缺失 ,差异具有显著性 ( P<0 .0 5)。 1例低级别的脑胶质瘤、3例高级别的脑胶质瘤发生了 p1 5基因 5′CPG岛甲基化。结论　 p1 5基因异常可能参与脑胶质瘤的发生、恶性进展。基因纯合缺失是脑胶质瘤中 p1 5基因失活的主要机制更多还原

【Abstract】 Objective To investigate the abnormality of p15 gene in brain glioma and the correlation or it with occurrence or malignant progression of brain glioma.Methods Deletion and 5′CPG island methylation of p15 gene were detected by methods of PCR and PCR based methylation in 56 cases of brain glioma.Results Out of the 43 cases of high grade glioma,14 cases were found to have homozygous deletion of p15E1,while none of the 13 cases of low grade glioma was found to have deletion of p15E1 (P<0.05) .Methylation of 5 ’CPG island of p16 gene was found only in four casses of glioma.Conclusion Abnormality of p15 gene may involved in the occurrence and malignant progression of brain glioma.Homozygous deletion of gene is the major mechanism of inactivation for p15 gene in brain glioma.更多还原