【摘要】 目的　研究 p1 6基因在人肺癌中表达和 DNA倍体的关系及意义。方法　以免疫组化S- P法检测 82例肺癌组织中 p1 6蛋白表达 ,用图像分析仪测定 DNA倍体。结果　 p1 6在肺癌表达阳性率为 51 .2 % ,低于癌旁组织 (P<0 .0 5) ,腺癌 p1 6阳性率最高为 68.8% ,高于鳞癌及未分化癌(P<0 .0 5)。在有淋巴结转移和无淋巴结转移组间 、 期与 、 期两组间 ,p1 6表达阳性率差异均有显著性 (P<0 .0 1 )。同时发现二倍体 p1 6阳性率显著高于异倍体 (P<0 .0 5)。结论　 p1 6基因异常与肺癌发生发展有关 ,在不同组织类型肺癌发生中的作用不同 ,且与肺癌临床分期及恶性程度有关。更多还原

【Abstract】 Objectives To study the relation and significance among tumor suppressor gene p16 expression and cellular DNA content in lung cancer.Methods The expression of p16 protein was determined by immunohistochemical method.The cellular DNA content was assayed by imagine analysis system.Results In 82 patients examined,51.2% showed p16 positive staining and p16 expression levels in adenocarcinoma exhibited higher than those in squamous cell carcinomas and small cell carcionomas( P<0.05 ).There were significantly difference respectively of the expression levels of p16 between lymph node metastasis group and no lymph node metastasis group and in groups of different clinical stages ( P<0.01 ).p16 positive staining rate in diploid was higher than that in aneuploidy.Conclusion Gene p16 play an important role in carcinogensis and progression of lung cancer and that they also correlate with the biological behavior and prognosis of lung cancer.更多还原