Super-distant molecular hybridization of plant seeds by ion beam-mediated gene cluster

【摘要】 <正> The N+ beam-mediated distant molecular hybridization between Ginkgo biloba L and watermelon was studied. The results showed that the ester gene of Ginkgo biloba L was successfully expressed in two varieties of watermelon, 3-16 and SR2-l4-2, in both of which the ester quantities were measured as 17.0756 μg/g and 45.9998 μg/ g respectively. Meanwhile, superoxide dismutase (SOD) activity in leaves of the watermelon expressing ester gene was increased twofold as compared to that of the control, showing that ion beam could mediate distant and/or super-distant donor gene expression in the cells of a receptor. Furthermore, the molecular mechanism of distant hybridization was analyzed.更多还原

【Abstract】 The N+ beam-mediated distant molecular hybridization between Ginkgo biloba L and watermelon was studied. The results showed that the ester gene of Ginkgo biloba L was successfully expressed in two varieties of watermelon, 3-16 and SR2-l4-2, in both of which the ester quantities were measured as 17.0756 μg/g and 45.9998 μg/ g respectively. Meanwhile, superoxide dismutase (SOD) activity in leaves of the watermelon expressing ester gene was increased twofold as compared to that of the control, showing that ion beam could mediate distant and/or super-distant donor gene expression in the cells of a receptor. Furthermore, the molecular mechanism of distant hybridization was analyzed.更多还原