【摘要】 目的 :比较 Brown Norway(BN)和 L ewis(L W)大鼠外周血白细胞产生肿瘤坏死因子 -α(TNF-α)和一氧化氮 (NO)的能力以及对氯化汞反应的差别。方法 :分离培养两种品系大鼠的外周血白细胞 ,加入 0 .0 1～ 1 0 .0 μmol/ L 的氯化汞 ,接触 1 2～ 96 h后 ,测定培养液中 TNF-α和亚硝酸盐的含量及 L DH的相对活性。结果 :氯化汞在 1 .0 μmol/ L 以下培养 72 h以内对大鼠白细胞无明显的细胞毒性。 BN品系白细胞产生 TNF-α的量明显高于 L W品系 ,而亚硝酸盐的生成量没有明显的品系差异。氯化汞能抑制两种品系大鼠的白细胞产生 TNF-α和 NO。结论 :两种品系大鼠白细胞体外生成 TNF-α的量存在差异 ,氯化汞对 TNF-α和 NO的生成都有抑制作用。更多还原

【Abstract】 Objective:To compare the strain differences of tumor necrosis factor alpha (TNF α) and nitric oxide(NO) production by leukocytes from Brown Norway (BN) and Lewis (LW) rats.Methods:Rat leukocytes were exposed to a series concentrations of HgCl 2(0.1～10.0 μmol/L)for different times (12～96 h).The relative lactate dehydrogenase (LDH)activities in the medium were measured to indicate the cytotoxicity.The TNF α concentration was determined by ELISA method and the nitrite was detected with Griess regent.Results:The HgCl 2 was not cytotoxic to the leukocytes at 1.0 μmol/L within 72 h of incubation.The concentrations of TNF α were signi ficantly higher in BN strain than those in LW strain in all the observation intervals,while the nitrite released from the two strains were not significantly different.HgCl 2 inhibited the production of TNF α and NO in a concentration dependent manner.Conclusion:There is a strain difference in the production of TNF α, but not NO,by the leukocytes from BN and LW rats.HgCl 2 can inhibit the production of TNF α and NO in both strains.更多还原