【摘要】 目的　研究异种黑色素细胞疫苗对小鼠恶性黑色素瘤生长的抑制作用。方法　制备异种黑色素细胞疫苗 ,皮下预防免疫小鼠后再接种恶性黑色素瘤 ,并以该疫苗皮下注射治疗恶性黑色素瘤鼠 ,随后测量肿瘤大小。采用51Cr释放法 ,体外分析小鼠脾细胞的细胞毒性T细胞 (CTL)活性 ,以间接ELISA法检测小鼠血清抗肿瘤抗体。结果　预防免疫使 90 %小鼠恶性黑色素瘤生长受到抑制 ,疫苗治疗使 5 0 %小鼠肿瘤生长受到抑制 ,免疫组鼠较对照组CTL杀伤活性在 2 0∶1、10∶1、5∶1不同效靶比时 ,分别增高 34 0 %、2 4 1%及 13 9% (P <0 0 5 ) ,体内出现抗恶性黑色素瘤抗体。结论　异种黑色素细胞疫苗可诱导小鼠抗恶性黑色素瘤特异性免疫反应 ,抑制肿瘤生长。更多还原

【Abstract】 Objective To study the inhibition of melanoma growth in mice by vaccination with xenogeneic melanocytes. [WT5”HZ]Methods Xenogeneic vaccine was prepared from pig eye melanocytes. It was used before or after B16 melanoma challenge in C57 mice. The size of tumor was monitored. Cytotoxic T lymphocyte (CTL) activity of mouse spleen cells was measured by 51Cr release assay. Antibody response against pig melanocytes and B16 melanoma cells were detected by indirect ELISA. [WT5”HZ]Results Preventive vaccination resulted in inhibition of tumor growth in 90% of the immunized mice, while therapeutic vaccination inhibited tumor growth in 50% of the treated mice. Specific CTL activity and antibodies in the immunized mice were detected. Conclusion[WT5”BZ] Antitumor immune response capable of inhibiting melanoma growth can be induced by xenogeneic melanocyte vaccination.更多还原