【摘要】 目的　探讨原发性食管鳞状细胞癌中血管内皮生长因子 (VEGF)的表达情况及其与肿瘤血管生成和预后的关系。方法　采用免疫组织化学染色方法 ,分别以抗VEGF多克隆抗体和抗F VIII单克隆抗体检测 82例手术切除标本中VEGF的表达水平和肿瘤微血管密度 (MVD)值 ,分析二者之间及与患者预后的相关性。结果　全组病例中 ,肿瘤细胞VEGF阳性率为 6 3 4% (5 2 / 82 )VEGF阴性和VEGF阳性的 3年和 5年生存率分别为 5 3 4%和 46 6 % ;19 2 %和 11 5 %。MVD中位值为37个 /mm2 (9～ 15 0个 /mm2 )。VEGF的表达水平与MVD值密切相关 (P =0 0 0 1)。MVD与患者的预后显著相关 (P =0 0 17)。随着VEGF表达水平增高 ,患者预后明显恶化 (P =0 0 0 0 )。结论　VEGF在食管鳞状细胞癌的肿瘤血管生成过程中发挥重要的调控作用。多因素预后分析表明VEGF的表达可作为食管鳞状细胞癌患者独立的预后判断指标。更多还原

【Abstract】 Objective To study the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in primary esophageal squamous cell carcinoma and to clarify the association of VEGF expression with the angiogenesis and prognosis of this tumor. Methods VEGF expression and MVD in 82 specimens of primary esophageal squamous cell carcinoma, resected from Jan 1981 through May 1994, were examined by immunohistochemistry staining (SP method), using anti VEGF polyclonal antibody and anti factor Ⅷ monoclonal antibody. The relationship between VEGF expression and MVD and postoperative survival of patient was analyzed. Results Fifty two out of the eighty two specimens of esophageal squamous cell carcinoma (63.4%) showed positive expression of VEGF. The median of MVD in tumor was 37/mm 2 (9～150/mm 2). There was a close correlation between MVD and VEGF expression ( P =0.001). The survival rate of patients with high MVD in the tumor was significantly lower than that of patients with low MVD ( P =0.017). The higher the level of expression of VEGF in tumor, the poorer prognosis for the patients ( P =0.000). Conclusion In primary esophageal squamous cell carcinoma, angiogenesis is mediated mainly by VEGF. VEGF is associated with tumor progression and may increase the malignancy via angiogenesis. It may be used as an independent indicator for prognosis of esophageal squamous cell carcinoma.更多还原