【摘要】 目的　比较霉酚酸、地塞米松对血管内皮细胞血管生成能力的影响 ,进一步探讨上述免疫抑制剂抗炎作用的机制。方法　以体外培养的人脐静脉来源的内皮细胞为研究对象 ,利用体外血管生成体系观察 2种免疫抑制剂对内皮细胞血管生成作用的影响 ,同时利用划伤愈复测定、3 H TdR掺入率及粘附能力测定实验分别观察其对内皮细胞迁移、增殖和粘附能力进行了测定。结果　霉酚酸酯能明显抑制血管内皮细胞血管生成。此外霉酚酸酯还表现出对血管内皮细胞增殖和迁移能力的抑制能力 ,而地塞米松对此则无显著影响 ;对于粘附能力 ,2种免疫抑制剂均未表现出显著的效应。结论　在本研究条件下霉酚酸较地塞米松表现出更强的抑制血管内皮细胞血管生成的作用。霉酚酸的上述特点可能是其在多种伴血管炎改变的自身免疫性疾病中有较显著疗效的机制之一更多还原

【Abstract】 Objective To explore the effect of mycophenolic acid (MPA),metabolite of mycophenolic mofetil (MMF) in human body, and dexamethasone (Dexa) on angiogenic activity of human umbilical vein endothelial cells (HUVEC) and the mechanisms of the anti inflammatory function of these two immunosuppressants. Methods A cell line of HUVEC was cultured in collagen gel. bFGF and VEGF were added to induce the angiogenesis. MPA and Dexa were added respectively. The capillary like structure formation was observed by microscopy. Scratch wound closure assay was performed to measure their effect on the migration activity of HUVEC. The ability of proliferation of HUVEC was determined by 3H TdR incorporation assay. The number of cells attached to the wall was counted by microscopy to observe the adhesion ability of HUVEC. Results MPA and Dexa, especially the former, inhibited the angiogenesis of HUVEC in vitro. MPA inhibited the migration and proliferation ability of HUVEC significantly, and Dexa did not show similar effect. Both of the two immunosuppresants could not inhibit the adhesion function of HUVEC. Conclusion MPA and Dexa, especially the former, inhibit the angiogenic ability of endothelium. This effect might explain the effect of MMF in the treatment of diseases with immune mediated vasculitis.更多还原