【摘要】 目的　研究视网膜母细胞瘤 (retinoblastoma ,Rb)细胞系HXO Rb4 4细胞多药耐药基因(multidrugresistancegene ,MDR1)和多药耐药相关蛋白 (multidrugassociatedprotein ,MRP)基因的表达 ,探讨Rb多药耐药现象的发生机制。方法　用逆转录聚合酶链反应检测HXO Rb4 4细胞的MDR1和MRP基因的表达 ,并用免疫组织化学方法对其蛋白产物P gp和P190进行检测。结果　HXO Rb4 4细胞系同时存在MDR1和MRP基因 ,并呈现P gp和P190的过度表达 (96 %和 97% )。结论　MDR1和MRP基因的过度表达是Rb多药耐药现象发生的重要机制。更多还原

【Abstract】 Objective To investigate the expression of multidrug resistance gene (MDR1) and multidrug associated protein (MRP) gene in the retinoblastoma (Rb) cell line HXO RB 44 , and study the mechanism of multidrug resistance (MDR) phenotype occurring in retinoblastoma. Methods Reverse transcripase polymerase chain reaction (RT PCR) was employed to detect the transcription of MDR1 and MRP gene in the HXO RB 44 cell line, and immunohistochemical technique was used to detect their protein products P glycoprotein and multidrug resistance associated protein (P190). Result Both MDR1 and MRP genes were expressed in HXO RB 44 cell line, as well as P glycoprotein and P190 were over expressed (96% 97%) in this cell line. Conclusion Over expression of MDR1 and MRP genes is significantly involved in the mechanism of MDR phenotype in retinoblastoma.更多还原