【摘要】 目的　探讨抗癌剂、活性氧、凋亡三者之间的关系 ,从而进一步探讨抗癌剂的作用及肿瘤细胞耐药机制以及活性氧在肿瘤发生上的意义。方法　用足叶乙甙 (Vp16 )、阿霉素 (ADR)、顺铂(DDP)作用于K5 6 2细胞 ,用流式细胞仪检测三种抗癌剂在不同浓度、作用 2 4h对K5 6 2细胞活性氧产生的影响及对凋亡的影响。同时用形态学方法观察凋亡细胞的形态改变。结果　①Vp16、ADR、DDP三种抗癌剂均可通过激发K5 6 2细胞产生活性氧来诱导该细胞发生凋亡 ;②每种抗癌剂激发K5 6 2细胞产生活性氧及诱发该细胞凋亡各有其最适浓度 (Vp16为 5 μg/ml,ADR为 3μg/ml,DDP为 5 μg/ml)及最佳作用时间 (2 4h)。结论　流式细胞仪 (FCM)测定细胞内活性氧产生状态及细胞凋亡情况 ,方法具有敏感、快速、简便、只需微量血、可除去坏死细胞碎片等优点。可用于抗癌剂的筛选 ,指导临床用药及选择敏感抗癌剂的有效剂量及最佳时间 ,并为探索治疗肿瘤新方法提供一个思路更多还原

【Abstract】 Objective To investigate the relationship between apoptosis (AP) and fluorescent intensity of reactive oxygen species (ROS) in cancer cell lines induced by anticancer agents, and the mechanisms of drug sensitivity and resistance. Method K562 cells were treated respectively with each of the Vp16, ADR and DDP, the fluorescent intensity of ROS and AP percentage of K562 cells at different concentration of the drugs treated were assayed by flow cytometry (FCM). Result ①After treatment with these drugs for 24 hours, the fluorescent intensity of ROS and AP percentage of K562 cells increased obviously as compared with that of control groups. ②The optimum concentrations of the drugs for ROS production and AP of K562 cells were determined. Conclusion Anticancer agents induced AP of K562 cells, which might be through stimulating ROS production.更多还原