【摘要】 目的　探讨普通重力和模拟微重力条件下构建许旺细胞三维培养体系的方法。　方法　对聚羟基乙酸细丝支架进行化学改性处理后再以层粘连蛋白进行生物学修饰 ;分别在普通重力和模拟微重力条件下把原代许旺细胞悬液接种在支架材料上 ,观察许旺细胞的粘附生长情况。　结果　两种重力条件下许旺细胞在聚羟基乙酸支架表面都能良好贴壁生长 ,培养 2 d时细胞生长稳定、密度适中 ;模拟微重力条件下许旺细胞在支架表面的分布更均匀。　结论　普通重力和模拟微重力环境都适宜构建许旺细胞的三维培养体系 ,模拟微重力环境可能更为有利。更多还原

【Abstract】 Objective To develop Schwann cells(SC) culture systems for three dimensional modeling under regular gravity and simulated microgravity Methods Primary Schwnn cells were used to seed polyglycolic acid fibers which were chemicalized and then coated with laminin (LN) under regular gravity and simulated microgravity Results Primary SCs attached excellently to the PGA fibers under both regular gravity and simulated microgravity SCs became stable and their density on the scaffolds was suitable 2 days after seeding SCs distributed more uniformly under simulated microgravity Conclusion Both regular gravity and simulated microgravity are suitable to develop Schwann cells culture systems for three dimensional modeling,and the latter may be better更多还原