【摘要】 目的　了解甲型肝炎病毒 (HAV)在中国几个城市的基因型分布 ,为HAV的分子流行病学追踪调查提供方法和依据。方法　 17株HAV代表株分别来自不同城市的甲型肝炎病人粪便或血清 ,病毒RNA经蛋白酶K消化、酚 /氯仿提取和乙醇沉淀后 ,以逆转录 套式聚合酶链反应 (RT PCR)扩增合成HAVVP1/ 2A交接区基因区 ,并进行直接核苷酸序列分析和差异比较。结果　VP1/ 2A交接区核苷酸序列分析表明 ,所有病毒株均从属于基因Ⅰ型 ;约 53%为ⅠB亚型 ,亚型间差异小于6 % ;约 4 7%为ⅠA亚型 ,亚型间差异小于 5 3% ;ⅠA与ⅠB亚型间的同源性为 88 7% - 92 3%。结论　中国流行或散发的HAV株可能有基因ⅠA、ⅠB亚型同时存在 ,流行病学相关的病毒株核苷酸序列相同或相近。更多还原

【Abstract】 Objective To clarify the distribution of hepatitis A virus (HAV) genotype in geographical regions of China Methods Seventeen representative HAV strains were isolated from the stool or serum of hepatitis A patients in different geographical regions Viral RNA was recovered from stool or serum by proteinase K digestion and phenol chloroform extraction, followed by ethanol precipitation prior to reverse transcription and polymerase chain reaction (RT PCR) amplification The nucleotide sequences of VP1/2A junction region were tested by using a direct sequencing technique Results A pairwise comparison of sequences within 168 bases at the VP1/2A junction revealed that all the sequences clustered within genotype Ⅰ About 53% of strains clustered in genotype ⅠB, with less than 6% variability; while the others clustered in genotype ⅠA, with less than 5 3% variability Sequence homology between genotype ⅠA and ⅠB varied from 88 7% to 92 3% Conclusion Epidemic or sporadic HAV strains in China may belong to HAV genotype ⅠA or ⅠB Epidemiologically related strains may be identical or closely related in sequence更多还原