【摘要】 目的　探讨供者肝脏非实质细胞输注诱导受者特异性免疫耐受的效果。方法　将 2×1 0 7个C3H/HE小鼠的肝脏非实质细胞通过尾静脉输入C57BL/ 6小鼠的体内 ,4 8h后给其腹腔注射环磷酰胺 2 0 0mg/kg ,1 8d后接受C3H/HE小鼠的皮片移植 ,观察皮片的存活情况。于肝脏非实质细胞输注前及输注后 1 8d、30d、6 0d进行供、受者混合淋巴细胞培养。结果　 1 5只输注C3H/HE小鼠肝脏非实质细胞的C57BL/ 6小鼠 ,其移植皮片的存活时间明显延长 ( 74 .6d± 9.7d ,对照组仅存活 1 0d± 0 .4d) ;混合淋巴细胞培养的结果证实受者的T淋巴细胞对供者小鼠淋巴细胞的反应程度明显降低。结论　肝脏非实质细胞可以有效诱导免疫耐受。更多还原

【Abstract】 Objective To investigate the effect of donor liver nonparenchymal cells (NPC) in the induction of tolerance by pretransplant infusion of it. Methods C57BL/6(B6) mice received the intravenous injection of 2×10 7 liver non parenchymal cells via a tail vein in C3H mice. After 48?h, cyclophosphamide in PBS was injected intraperitoneally into B6 mice in a dose of 200?mg/kg. Eighteen days later, B6 mice received C3H mice skin graft transplant. Mixed lymphocytes culture(MLC)was performed on the different post intravenous time points. Results The survival time of skin graft in 15 mice was prolonged obviously with the mean survival time being 70± 17.2 ?days (the mean survival time of normal control was only 10±4?days). MLC proved that the reaction capacity of T cell of B6 mice to donor was decreased significantly. Conclusions The donor specific immune tolerance could be successfully induced by the NPC infusion combined with short term immune suppression-cyclophosphamide therapy.更多还原