【摘要】 目的　探讨铜绿假单胞菌生物被膜 (BF)的耐药机制。方法　用改良平板培养法在硅胶膜上建立铜绿假单胞菌BF模型。用银染法及扫描电镜对BF进行鉴定。用紫外分光光度法及Bio Rad蛋白定量试剂法检测铜绿假单胞菌的浮游细菌 (A组 )、BF菌 (B组 )及亚胺培南诱导BF菌 (C组 )产生 β 内酰胺酶的能力。 结果　B组产生 β 内酰胺酶的活性及产酶量高于A组 ,分别是A组的 4 6 7倍和 2 0 9倍 ,C组产酶活性及产酶量高于A组 ,分别是A组的 2 1 86倍和 6 2 8倍 ,也高于B组 ,是B组的 4 6 8倍和 3 0 0倍。t检验结果 :A、B、C 3组产 β 内酰胺酶活性及产酶量两两之间比较P值均 <0 0 1,表明各组之间产 β 内酰胺酶的活性及产酶量具有显著差异。 结论　BF铜绿假单胞菌的耐药与其产生 β 内酰胺酶有重要关系。更多还原

【Abstract】 Objective To analyze the resistance mechanism of Pseudomonas aeruginosa biofilm bacteria. Methods An in vitro model of Pseudomonas aeruginosa bacterial biofilm was established in silicon disk with modified flat board method and a rapid staining procedure of AgNO 3 was used to verify it. Biofilm was observed under scanning electron microscopy. The test was carried out in three groups. Group A was planktonic bacteria of Pseudomonas aeruginosa , group B biofilm bacteria of Pseudomonas aeruginosa and group C biofilm bacteria induced by imipenem. The activity of β lactamase was quantitated with a spectrophotometric assay method and β lactamase quantitation was determined by using Bio Rad protein assay. Results The activity and protein content of β lactamase in group B was higher than that in group A by 4.67 and 2.09 times. The activity of group C was 21.86 times as much as that in group A and 4.68 times as much as that in group B. The quantitation in group C was 6 28 times as much as that in group A and 3.00 times as much as that in group B . The activity and quantitation of the three groups were different significantly from each other ( P <0.01). Conclusion The production of β lactamase in Pseudomonas aeruginosa biofilm bacteria is one of the main reasons of its resistance.更多还原