【摘要】 目的　研究产前缺氧性适应对新生大鼠脑组织是否具有保护作用。方法　将临产前孕鼠 (孕期 2 2d) 12只随机分成 2组 ,Ⅰ组为实验组 (缺氧性适应组 ) ,Ⅱ组为对照组。Ⅰ组 :将孕鼠置于密封仓中 ,当氧浓度降至 15 %时 ,将孕鼠放入新鲜空气中 5min ,再放入密封仓中 ,重复第一次过程 ,然后待其自然分娩。Ⅱ组 :除密封仓不密闭外 (氧浓度 2 1% ) ,其余同Ⅰ组。将两组娩出的新生鼠分别随机取出 4 0只 ,做新生鼠缺血缺氧性脑损伤模型 ,随机取两组的新生大鼠各 30只于脑缺血缺氧后2 4h处死 ,取脑组织分别进行光镜、电镜和流式细胞仪检测。结果　Ⅰ组 (缺氧性适应组 )神经细胞多为正常 ,细胞排列较整齐 ,有少许早期凋亡细胞 ,凋亡率 (2 9%± 1 2 % )。Ⅱ组 (对照组 )神经细胞排列紊乱 ,部分细胞胞体肿胀 ,少部分呈空泡状 ,可见早期和中期凋亡细胞 ,少许晚期凋亡细胞和坏死细胞 ,凋亡率 (9 5 1%± 3 6 2 % ) ,凋亡率两组有统计学差异。结论　孕鼠产前缺氧性适应可以明显降低新生鼠缺血缺氧性脑损伤程度 ,对新生大鼠脑组织具有明显的保护作用更多还原

【Abstract】 Objective To examine whether the prenatal hypoxia adaptation has any protective effect on the brain of the newborn rat Methods 12 22 day pregnant Wistar rats were randomly divided into 2 groups: the control group and the treated group In the treated group the pregnant rats were placed in a tightly closed hypoxia adaptation chamber When the O 2% in the chamber dropped to 15%, the rat was taken out to breathe fresh air for 5 min then put back in the chamber This process was repeated until its natural delivery In control the chamber was not tightly closed (O 2%=21%) 40 newborn rats weighing 6 8g were selected and subjected to brain ischemia and hypoxia, Left common carotid artrey was ligated under ether anesthesia 2h after recovery from surgery the newborn rats were placed in hypoxia chamber (T=36℃±1℃,O 2%=9%)for 1 5h 24h later they were sacrificed and brain was removed for microscopic examination (optical and electron) and flow cytometry measurement Results In the treated group most newborns were normal There were a few apoptosis cells in early stage The rate of apoptosis was 2 9%, necrosis cells could hardly be seen In the control group, although most neurons were also normal but there were apoptosis cells in early, middle and late stage and even necrosis cells The rate of apoptosis was 9 51%, which was significantly different from that in the treated group Conclusions Prenatal hypoxia adaptation has protective effect on the brain of newborn rat更多还原