【摘要】 目的 观察不同时间异丙酚预处理对沙上鼠脑缺血再灌注损伤的保护作用。方法 沙土鼠４２ ．只，随机分为对照组、缺血损伤组、异丙酚预处理组（脑缺血前４８ｈ、２４ｈ、１２ｈ、６ｈ、１ｈ各组分别以异丙酚１００ ｍｇ·ｋｇ－１腹腔注射）等７组，观察指标为脑组织内皮素（ＥＴ）、降钙素基因相关肽（ＣＧＲＰ）、丙二醛（ＭＤＡ）含量以及超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ－ｐｘ）活性的改变并制作电镜标本行透射电镜观察。结果 异丙酚预处理各组 ＥＴ含量、ＥＴ／ＣＧＲＰ含量的比值及ＭＤＡ含量明显低于缺血损伤组（Ｐ＜０．０５），而ＣＧＲＰ含量和ＳＯＤ、ＧＳＨ－ｐｘ活性明显高于缺血损伤组（Ｐ＜０．０５），其中尤以异丙酚预处理２４ｈ组的ＥＴ及ＥＴ／ＣＧＲＰ值降低更明显，而ＣＧＲＰ值明显高于其余异丙酚预处理组（Ｐ＜０．０５）；电镜结果也显示异丙酚预处理２４ｈ组脑组织的超微结构改变最小。损伤最轻。结论 缺血前４８ｈ至缺血前１ｈ各时点异丙酚预处理对沙土鼠缺血性脑损伤均有不同程度的保护作用，尤以缺血前２４ｈ给药对沙土鼠脑缺血再灌注损伤的保护作用优于其它各时点异丙酚预处理组。更多还原

【Abstract】 Objective To assess the protection of propofol pretreatment against cerebral ischemiareperfusion injury in gerbils. Methods Forty-two Mongolian gerbils of both sex, aged 3 months, weighing 50-70 g were randomly divided into seven groups of six animals each:(A) control group, sham operation;(B) ischemia group;(C-G) propofol groups, propofol 100mg/kg was given intraperitoneally(ip) 48 h(C), 24 h(D), 12 h(E), 6 h(F) and 1 h(G) before ischemia. In group B-G duration of ischemia was 20 mm and duration of reperfusion 50 mm. Animals were anesthetized with pentobarbital 36 mg/kg ip and bilateral common carotid arteries were exposed and clamped for 20 mm and then unclamped for reperfusion which lasted for 50 mm. The animals were then decapitated and forebrain cerebral cortex was removed for determination of endothelin(ET), calcitonin gene-related peptide(CGRP) and malondialdehyde(MDA) contents and activities of superoxide dismutase(SOID) and glutathione peroxidase(GSH-px) in addition to electronic microscopic examination. Results In propofol groups ET content, ET/CGRP and MDA level were significantly lower than those in ischemia group(P< 0.05) and CGRP content and SOD, GSH-px activities were significantly higher than those in ischemia group(P ＜ 0.05＝, especially in group D(propofol was given ip 24 h before ischemia of brain). In group D ET content and ET/CGRP were the lowest and CGRP value the highest among the five propofol groups. Electronic microscopic examination also showed that there was no obvious distinction in super subtle structure of brain between control group and group D.Conclusions Propofol given 1-48 h before cerebral ischemia affords varying degree protection against cerebral ischemia-reperfusion injury. The best protection is provided by propofol given 24 h before cerebral isehemia.更多还原