【摘要】 目的　探讨多聚左旋赖氨酸 (PL)对软骨形成的促进作用及其机制。方法　用含不同剂量PL( 0～ 15 μg/ml)的培养液对大鼠肢芽细胞培养 4d ,根据培养细胞中软骨集落形成情况和Ⅱ型胶原的表达情况 ,判断PL对软骨形成的促进作用 ;采用免疫组化方法检测神经钙黏附蛋白的表达 ,探讨PL促进软骨形成的机制。结果　PL以剂量依赖性方式促进培养细胞中软骨集落的形成 ,情况如下 :5 9± 13( 0 μg/ml) ;82± 14 ( 1μg/ml) ;97± 19( 3μg/ml) ;10 8± 2 4( 5 μg/ml) ;软骨细胞集落互相连接成片状 ,以至未能彼此区分 ( 10 μg/ml) ;软骨细胞集落形成减少 ,且不典型 ( 15 μg/ml)。免疫组化实验结果显示 :高剂量PL组 ( 5 μg/ml)中Ⅱ型胶原和神经钙黏附蛋白的表达较低剂量PL组 ( 0 μg/ml)增强。 结论　PL可促进软骨形成且可能是通过增强神经钙黏附蛋白的表达来完成的更多还原

【Abstract】 Objective To investigate the effect and possible mechanism of poly L lysine (PL) on promoting chondrogenesis in vitro. Methods High density cultures of rat limb bud cells were exposed to culture media containing PL (0 ～15 μg/ml) and cultured for four days. Chondrogenesis was assayed based on cartilage nodule number and expression of type Ⅱ collagen. The possible mechanism of the promotion of chondrogenesis in vitro by PL was analyzed by examining neural cadherin (N cadherin) expression through immunohis to chemistry. Results Compared with untreated cultures, PL treated cultures contained a significantly elevated number of cartilage nodules in a dose dependent manner,which was as follows: 59±13 (0 μg/ml);82±14 (1 μg/ml);97±19 (3 μg/ml);108±24 (5 μg/ml);By 10 μg/ml of PL, chondrogenesis was stimulated to such an extent that nodules which confounded with each other were connected to be a flat;Cultures treated with 15μg/ml of PL produced less detectable nodules. The immunohis to chemistry showed that expression of type Ⅱ collagen and N cadherin were increasing in PL treated cultures (5μg/ml). Conclusion PL promotes chondrogenesis in vitro, which may be achieved by enhancing the expression of N cadherin.更多还原