【摘要】 目的　探讨先天性肾积水转化生长因子 β1(TGF β1)mRNA及蛋白表达变化的临床意义。　方法　应用免疫组化及原位杂交方法对 15例患肾、10例正常肾组织进行染色观察。　结果　患肾组织TGF β1蛋白及mRNA表达均增高 ,大部分近曲小管、少数肾小球、极少数远曲小管细胞浆内可见TGF β1蛋白强阳性表达。大部分近曲小管的部分细胞核、极少数肾小球细胞核内可见TGF β1mRNA的中度阳性表达。患肾肾盂及肾盂输尿管连接处狭窄段肌细胞和间质梭形细胞胞浆及胶原纤维内TGF β1蛋白及mRNA表达增高。结论　先天性肾积水患肾组织TGF β1表达增高可能与慢性肾损伤及间质纤维化有关更多还原

【Abstract】 Objective To detect the expression of TGF - β 1 mRNA and TGF-β 1 protein in children with hydronephrosis and to evaluat e the role of TGF-β 1 in chronic renal damage in congenital hydronephrosis. Methods The expression of TGF-β 1 mRNA and TGF-β 1 protein in renal parenchyma, renal pelvis and pelvic ureteral junction from ch ildren with congenital hydronephrosis were studied by immunohistochemistry and in situ hybridization. Results The expression of TGF-β 1 increased in the affected renal parenchyma, pelvis and PUJ. The expression o f TGF-β 1 protein was mainly localized in the plasma of proximal tubular cell s. There was also positive expression in a few renal glomerular cells and distal tubular cells. The expression of TGF-β 1 mRNA was mainly distributed in the cell nucleus of proximal tubular cells. Positive granules were also seen in a fe w renal glomerular cells.The expression of TGF-β 1 mRNA and TGF-β 1 prote in was enhanced in the muscle cells, spindle cells and collagenous fibers in the pelvis and PUJ of the affected kidney. Conclusions The increase of TGF-β 1 expression in the kidney with congenital hydronephrosis might related to the chronic renal damage and renal interstitial fibrosis.更多还原