【摘要】 目的　探讨i(17q)和双微体 (doubleminutes ,DM )在膀胱移行上皮细胞癌中的表达及意义。　方法　正常对照组 15例 ,肿瘤术前组、术后组各 14例 ,采集术前、术后外周静脉血全血培养 ,常规染色体制备 ,检测i(17q)和DM的表达 ,并与临床病理因素及预后的关系进行分析。　结果　正常对照组无DM检出 ,肿瘤术前组和术后组分别检出 35对和 31对DM ,与对照组相比差别有显著性意义 (P <0 .0 1) ,且集中于 2例DM阳性病例 ,但术前组与术后组比较差别无显著性意义 (P >0 .0 5 )。其中病理级别最高的 1例患者手术后外周血出现同时具有i(17q)和DM的分裂相。 　结论　DM可作为癌基因扩增的一项独立的预后指标 ,DM阳性患者预后差 ,易复发。DM受累基因估计位于 17号染色体长臂上。更多还原

【Abstract】 Objective To investigate the expression of i(17q) and double minutes (DM) in bladder transitional cell carcinoma (TCC) patients. Methods 15 controls and 14 TCC patients were examined the whole blood sample for i(17q) and DM both before and after operation. Results DM was not found in all the controls whereas there were 35 pairs of DM found before operation and 31 pairs after operation in patients with TCC (P<0.01) especially dominantly shown in 2 patients.There was no significant diffe- rence between the pre-and post-operarive findings.The patient with the highest tumor grade had one metaphase showing i(17q) and DM at the same time after operation. Conclusions DM may be an independent prognosis parameter of cancer gene amplication denoting treatment failure and rapid recurrence.The DM related genes may be located at 17q.更多还原