【摘要】 目的　探讨良性前列腺增生时细胞增殖与凋亡变化的临床意义。　方法　收集正常前列腺和BPH移行区和周围区标本各 30例。应用Ki 6 7和bcl 2免疫组织化学染色对所有标本进行增殖与凋亡状态检测 ,并比较正常移行区和BPH移行区、正常周围区和BPH周围区、正常移行区和周围区、BPH移行区和周围区的表达情况。　结果　正常组周围区和移行区ki 6 7平均阳性细胞比例分别为 (4 .30± 1.0 3) %和 (4 .35± 1.11) %。周围区和移行区Bcl 2平均阳性细胞比例分别为 (2 .70± 1.30 ) %和 (2 .40± 0 .80 ) % ,两个区带间差别无显著性意义 (P >0 .0 5 )。BPH组周围区和移行区ki 6 7平均阳性细胞比例分别为 (4 .87± 1.2 3) %和 (9.83± 2 .41) % ,两个区带间差别有非常显著性意义 (P <0 .0 1) ;周围区和移行区Bcl 2平均阳性细胞比例分别为 (6 .45± 7.45 ) %和 (7.37± 2 .42 ) % ,两个区带间表达阳性细胞比例及染色强度差别无显著性差意义 (P >0 .0 5 )。两组周围区间ki 6 7的表达差别无显著性意义 (P >0 .0 5 ) ,但BPH中周围区Bcl 2表达高于正常前列腺周围区 (P <0 .0 5 )。BPH时移行区增殖活性较正常前列腺明显增高 (P <0 .0 1) ;同样上皮细胞凋亡率却显著下降 (P <0 .0 1)。　结论　BPH周围区的细胞增殖与正常前列腺?更多还原

【Abstract】 Objective To assay the expressions of ki 67 and bcl 2 in different zones of normal prostates and in prostates from BPH patients so as to assess the proliferation and apoptosis status. Methods Specimens from 30 normal subjects without prostatic disease and prostates from 30 BPH patients undergone Madigan prostatectomy were used for the study.Detection of ki 67 and bcl 2 in different zones of the prostates were undertaken so as to assess the proliferation and the apoptosis status. Results In the 30 normal prostate subjects with a mean age of 25,the mean positive cell percentage of ki 67 in the peripheral zone was (4.30±1.03)% and in the transitional zone (4.35±1.11)%.The mean positive percentage of bcl 2 in the peripheral zone was (2.70±1.30)% and in the transitional zone (2.40±0.80)%.There were no difference of both the two in the peripheral and the transitional zones ( P > 0.05 ).In the 30 patients with BPH with a mean age of 70,the mean positive cell percentage of ki 67 in the peripheral zone was (4.87±1.23)% and in the transitional zone ( 9.83 ± 2.41 )%. The mean positive cell index of bcl 2 in the peripheral zone was ( 6.45 ± 7.54 )% and ( 7.37 ± 2.42 )% in the transitional zone.The proliferation percentage of transitional zone was much higher than that in the peripheral zone ( P <0.01).Whereas,there was no significant difference of the apoptosis percentage between the 2 zones ( P >0.05).The proliferation percentage in the peripheral zone of BPH prostates was higher than that in normal prostates and the apoptosis percentage much lower in the transitional zone ( P <0.01.) Conclusions The proliferation percentage in the peripheral zone of BPH prostates was not significantly different from that in normal prostates while the apoptosis percentage in the peripheral zone of BPH prostates was much lower.The result also indicates that the proliferation percentage in the transitional zone is higher and the apoptosis percentage lower than those in the peripheral zone.All these may be associated with the pathogenesis of benign hyperplasia of the transitional zone.更多还原