【摘要】 目的　探讨生物被膜大肠杆菌的耐药机制。方法　用改进的平板培养法建立大肠杆菌生物被膜模型 ,用扫描电镜进行鉴定。分别检测浮游大肠杆菌 (A组 )、生物被膜大肠杆菌 (B组 )及亚胺培南 (C组 )和头孢西丁 (D组 )诱导后生物被膜大肠杆菌产 β内酰胺酶的活性。 结果　生物被膜大肠杆菌产 β内酰胺酶是浮游大肠杆菌的 2 16倍 ,经亚胺培南和头孢西丁诱导后生物被膜大肠杆菌 β内酰胺酶的活性分别是生物被膜大肠杆菌的 1 30倍和 1 0 5倍。结论　生物被膜大肠杆菌耐药与产生 β内酰胺酶有关更多还原

【Abstract】 Objective To investigate the mechanism of drug resistance of biofilm escherichia coli .Methods The model of escherichia coli biofilm was established with the flat board method And the biofilm was confirmed by scanning electron microscopy The β lactamase activities were quantitated in escherichia coli, biofilm escherichia coli , biofilm escherichia coli induced by impenem or cefoxitin. Results The β lactamase activity of biofilm escherichia coli was 2 16 times as much as that of escherichia coli planctonically, and the β lactamase activities of biofilm escherichia coli induceded by impenem or cefoxitin were 1 30 and 1 05 times as much as those of biofilm escherichia coli ,respectively.Conclusion The drug resistance to antibiotics of biofilm escherichia coli was related to the production of β lactamase.更多还原