【摘要】 目的　观察慢性阻塞性肺疾病 (COPD)缓解期腺病毒感染情况 ,探讨腺病毒在COPD发病中的作用。方法　采用PCR方法对 10例COPD患者 ,12例慢性支气管炎患者 ,6例支气管哮喘患者及 8名健康志愿者支气管上皮细胞及肺泡巨噬细胞DNA进行腺病毒早期转录单位 (EIA)基因检测。结果　 2 2例COPD与慢性支气管炎患者中检出E1A阳性 6例 ,占 2 7% ;其中COPD患者EIA阳性5例 ,占本组COPD患者的 5 0 % ;慢性支气管炎患者ElA阳性l例 ,占本组慢性支气管炎患者的 8% ;在支气管哮喘和健康志愿者均未检出EIADNA ,COPD组与慢性支气管炎组相比差异有显著性 (P <0 0 5 )。结论　在COPD稳定期存在腺病毒潜在感染 ,其在气流阻塞发生和发展的作用值得进一步研究更多还原

【Abstract】 Objective To study latent adenovirus infection in the stable stage of chronic obstructive pulmonary disease(COPD).Method The bronchial epithelial cells and alveolar macrophages from l2 patients with chronic bronchitis,10 patients with COPD,6 patients with asthma and 8 healthy volunteers were obtained by bronchosocpy. The adenoviral DNA was determined in the epithelial cells and macrophages. All patients were in the stable stage The polymerase chain reaction(PCR) was used for detection of the adenoviral genome E1A. Results the E1A region of adenovirus was found in the epithelial cells of COPD and chronic bronchitis patients (27%), not found in the patients with asthma and normal volunteers. Furthermore,E1A DNA was much more commonly in the COPD patients (50%) than in the patients with chronic bronchitis(8%)( P <0 05). Conclusions Latent adenoviral infection was present in the stable stage of COPD and may be related to the pathogenesis of the disease.更多还原