【摘要】 目的　研究哮喘豚鼠气道重塑的机制及气道反应性的变化。方法　实验分二组 :对照组 (2 0只 )和哮喘组 (2 0只 ) ,取双肺与不同刺激因素作用后作组织切片、HE染色 ,通过图像分析仪测定支气管内周长、管壁厚度、外周长 ,并计算平滑肌收缩百分比 (PMS)。结果　 (1)哮喘组支气管壁厚度 (WA/Pi)为 (10 0± 2 0 ) μm2 / μm ,支气管壁平滑肌厚度 (平滑肌的面积 /Pi)为 (4 8± 1 5 ) μm2 / μm ,对照组WA/Pi为 (7 9± 2 1) μm2 / μm ,平滑肌的面积 /Pi为 (3 1± 2 0 ) μm2 / μm ,两组比较差异均有显著性(P <0 0 1) ,支气管壁平滑肌细胞核数量 (N/Pi)哮喘组为 (0 0 12 3± 0 0 0 2 7)个 / μm ,对照组为 (0 0 10±0 0 0 3)个 / μm ,两组比较差异有显著性 (P <0 0 5 )。 (2 )哮喘组气道平滑肌对腺苷的反应性 (以PMS表示 )为 0 34± 0 0 7,对照组为 0 2 9± 0 0 8,两组比较差异有显著性 (P <0 0 5 ) ,联用氨茶碱和腺苷后 ,哮喘组PMS为 0 2 6± 0 0 7,与单用腺苷组 (0 34± 0 0 7)比较差异有显著性 (P <0 0 1)。 (3)在Ach作用下哮喘组的PMS为 0 2 4± 0 0 4,对照组为 0 19± 0 0 6 ,两组比较差异有显著性 (P <0 0 5 ) ,联用肝素和Ach后 ,哮喘组PMS为 0 2 0± 0 0 4,与单用Ach组更多还原

【Abstract】 Objective To observe the mechanism of airway remodeling and changes of airway responsiveness in guinea pig model of asthma. Methods 40 guinea pigs were randomly divided into two groups: control (20)and asthmatic group (20) After incubating with different stimulus, bilateral lung tissue section were stained with HE Using image analysis system to measure the airway internal perimeter, wall area, external perimeter, etc and calculate percentage of muscle shortening (PMS) according to formula. Results (1) The airway wall thickness (WA/Pi) in asthmatic group and control group were (10 0±2 0 )and (7 9±2 1) μm 2/μm, respectively The bronchial smooth muscle thickness in asthmatic group and control group were (4 8±1 5 )and (3 1±2 0) μm 2/μm, respectively Both were statistically significant ( P <0 01) The number of bronchial smooth muscle nucleus in asthmatic group (0 012 3±0 002 7/μm) was higher compared with control (0 010±0 003/μm) ( P <0 05) (2) Responsiveness of airway smooth muscle to adenosine (represented by PMS) in asthmatic group and control were 0 34±0 07 and 0 29±0 08,respectively ( P <0 05) When combined with aminophyline, PMS in asthmatic group was 0 26±0 07 There was statistically significant difference when compared with adenosine alone (0 34±0 07) ( P <0 01) (3) PMS to acetylcholine in asthmatic group and control were 0 24±0 04 and 0 19±0 06, respectively ( P <0 05) When combined with heparin, PMS in asthmatic group was 0 20±0 04 There was statistically significant difference when compared with acetylcholine alone (0 24±0 04 ) ( P <0 05) Conclusions The main reason of airway remodeling in asthma is due to bronchial smooth muscle hyperplasia Aminophylline and heparin may inhibit the responsiveness of airway to adenosine and acetylcholine respectively更多还原